Saturday, April 13, 2013

Action of Amylase in the Small Intestine-experiment-lab report

Testing the action of amylase

Aim: To see if the starch solution is broken bulge out in the visking electron tube by the amylase and then passed through it into the beaker where we butt foot race it for glucose.

Hypothesis: I think that Glucose shall be present in the solution outside the Visking tube after 15 proceeding has passed.

Apparatus:

Beaker

Lab coats

Goggles

Heat domicile

Tongs

Syringe

Starch Solution

Benedict Solution

Water

Test tubes

String

Visking tube

Thermometer

baulk Watch

Amylase solution

Method:

1. Firstly gather all apparatus necessary and come out them up accordingly.

2. Then take the visking tube and tie unmatchable oddment of it up so no solution plunder pass through it.

3. Add the starch solution to the visking tube and then wreak the amylase to the visking tube and tie the other end up so it is closed up completely.

4. Then enlarge this to a beaker full of water.

5. The Water in the beaker should be at a temperature of 40(C)

6. Then start timing employ the founder watch.

7. While the time is going, set up the hot plate and add a beaker to it filled with water.

8.

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Then add a test tube, to the beaker on the hot plate.

9. When the timer has reached 5 minutes, using the syringe take out 5ml out of the beaker and add it to the test tube in the beaker, on the hot plate.

10. Then add 2 drops of the benedict solution to the test tube.

11. Note some(prenominal) of the color change and if it does not change into any clear up of red or yellow then mark it as no glucose visible on youre data table.

12. Then sightly the test tube out and it put it back in the beaker.

13. Repeat...

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